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Calculate ic50 in prism5/15/2023 Personalization of cancer treatment is a major research effort aimed at identifying which patient is most likely to respond to a given treatment. The multilevel model performs significantly better in all cell lines. The CV statistics are shown for all the lines for both approached. The coefficient of variation was used to investigate the accuracy of estimates in a replicate series. The purple response is unexpected and is detected by the model error statistic root mean squared error. Illustration of responses for six randomly selected cell lines.įor each line a response with good activity (light blue), limited to no activity (dark blue) is shown. (E & F) show real fits and the underlying data for the same cell lines for three drugs. (D) A drug, cisplatin, illustrates how the characteristic response curve shifts (dashed lines) depending on its sensitivity, the black line is quite sensitive. (C) Two characteristic dose–response curves for two cell lines (see B) are plotted, with the population response in gray (A). Cell lines that are more resistant tend to have a shallow slope. (B) Cell lines differ in their position (x-axis) and slope (y-axis) parameter. (A) The population dose–response estimated by the model from the data. Graphical presentation of the model parameters. The inset (AUC) illustrates the area under the curve in the screening range, and is defined as A/(A + B).ĪUC: Area under the curve. Examples of a less potent drug (II) and a more potent drug (III) are shown with dashed lines. Changes to the center of the function define the change in IC 50, which is the concentration at which the relative viability is 0.5 (horizontal line). Each series spans 256-fold concentration range, and each unit translates to a twofold difference, meaning that 7 to 9 translates to a fourfold difference. Sigmoid dose–response relation for a mock compound.īlack line (I) shows the dose response function with a linear phase in the center of the sigmoid. First draft submitted: 6 October 2015 Accepted for publication: 2 February 2016 Published online: Figure 1.
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